thymus vulgaris fiche technique
The reported pharmacological activities of T. vulgaris L. include antibacterial, antioxidant, anti-inflammatory, antiviral, and anti-cancerous activities. Trouvé à l'intérieur â Page 218... antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. ... Use of innovative (micro)extraction techniques to characterize Harpagophytum procumbens root and its commercial ... The imbalanced expression of cytokines has been implicated in the progression of many diseases including CIBDs [31]. The supercritical extractions were carried out in a pilot-plant-scale supercritical fluid extractor (Thar Technology, Pittsburgh, PA, USA, model SF2000) of 2 L capacity using pure supercritical CO2 at a pressure of 300 bar and a temperature of 40°C. The aim of this study was to evaluate the effects of different gamma radiation doses (1, 5 and 10 kGy) on the individual profile of phenolic compounds determined by HPLC-DAD-ESI/MS, and the bioactive potential (cytotoxic, virucidal, and antimicrobial . The increase in protein secretion was used as an indicator for macrophage activation [3]. A Mediterranean aromatic plant, thyme, has been frequently used for those purposes. The essential oil of T. vulgaris L. isolated by hydro-distillation was of yellowish color, clear with an aromatic odor of thymol, in total yield of 1.5% (v/w) on dry . Discover our new products and get exclusive tips! The effect of Thymus vulgaris essential oil (TEO) and its isolated constituents thymol and cavacrol (CVL) were studied in the following experimental models: ear edema, carrageenan-induced pleurisy . Irradiation is a feasible and safe decontamination technique, being applied to several types of foods including edible and medicinal plants. Two oil fractions from each species were obtained by CO2 supercritical fluid extraction. Trouvé à l'intérieur â Page 229Thymol Thymol (2-isopropyl-5-methylphenol) is a major monoterpene phenol obtained from thyme (Thymus vulgaris) essential oil. Thymol binds with bacterial proteins and unsaturated fatty acids, disintegrates the cell membrane and enhances ... Properties of thyme extracts from three different species ( Thymus vulgaris , Thymus zygis, and Thymus hyemalis ) were examined. To harvest creeping thyme ground cover, either remove the leaves from the stems or dry by snipping from the plant and hanging upside down in a dark, well aerated area. Properties of thyme extracts from three different species (Thymus vulgaris, Thymus zygis, and Thymus hyemalis) were examined.Two oil fractions from each species were obtained by CO 2 supercritical fluid extraction. In this sense, SFE with CO2 is in increasing demand to produce high-quality essential oils from plant material with medicinal properties [26]. Thyme is tonic and energizing. human anatomy scientific illustrations: child thorax organs - thymus stock illustrations. Constituents were extracted from cell suspension after 40 days. Epub 2009 May 9. After differentiation, the cells were washed with PBS and treated with or without Cu2+ oxidized LDLs to activated or not activated them. These results were in agreement with the ones regarding the expression of cytokines genes at 24 hours of incubation. Trouvé à l'intérieur... respectively, using disc volatilization method. Thymus vulgaris L. (essential oil) Kohiyama et Aspergillus flavus MIC and MFC (minimum al. (2015) fungicidal concentration) were found at 250 μg/mL. Inhibition of the production of ... Its mildness connects it to the frontal chakra, but it is also quite active on the level of the umbilical center of energy. Mainly, atherosclerosis results from an immune response to oxidized low-density lipoproteins (LDLs) [3]. 2, 2019 pp. This perennial semi-evergreen shrub is used today as an aromatic plant to flavor food liqueurs and herb blends and as a medicinal plant for its antimicrobial and antioxidative properties to treat various diseases such as bronchitis and gastrointestinal distress. Thymol and CVL were isolated from TEO as fractions of hydrodistillated oil. J Appl Microbiol 1998;84(3):368-376. 2020 Jan 23;25(3):502. doi: 10.3390/molecules25030502. HPLC chromatogram of T. vulgaris essential oil with characteristic UV absorption spectra at…, MeSH Anti-inflammatory effect of thymol has been reported on human neutrophiles incubated with 10 or 20 μg/mL of this compound [14]. Abstract. Greek Thymos, “spiritedness, expressed passion”, is where the name thyme comes from. 2015 Jan-Feb;26(1):61-71. doi: 10.1002/pca.2537. Well known from amateurs as well as chefs, this herb holds an important place in Mediterranean cuisine. Le chémotype à linalol, doux, est caractérisé par sa bonne tolérance et sa facilité d'utilisation. How do you revive thyme that has died? The Raindrop Technique helps bring balance and harmony to the body by combining targeted massage and distinctive energy approaches. properties of Thymus vulgaris essential oil in topically applied formulations under a. challenge test. The cytotoxicity effect on THP-1 macrophages of S1 and S2 CO2 supercritical fluid extracts from thyme leaves was evaluated before the bioactivity study. 10 ng/μL total RNA was used as template for cDNA synthesis using the High-Capacity Archive Kit from Applied Biosystems, according to the manufacturer’s instructions. See this image and copyright information in PMC, A New Method for Determination of Thymol and Carvacrol in. Sous-arbrisseau ligneux. Fractionation conditions were as follows: separator 1 was kept at constant pressure and temperature of 15 MPa and 40°C, respectively, whereas separator 2 was maintained at a pressure of 2 MPa and a temperature of 40°C. Borneol, another compound present in thyme, has been also described as an anti-inflammatory since its dietary supplementation significantly decreases the concentration of the proinflammatory cytokines IL-1β and IL-6 in mice [5]. To determinate whether thyme oils could have immunomodulation properties and could mediate in inflammatory cytokines regulation, we study the effect of our extracts on ox-LDL-activated THP-1 macrophages, measuring the expression and release of several inflammatory mediators. Induced by an atherogenic diet, monocytes are promoted to adhesion into the artherial endothelium and intimae (diapedesis). Human THP-1 monocyte cell lines (American Type Culture Collection, ATCC) were maintained in suspension in RPMI 1640 culture medium (ATCC) supplemented with 10% FBS (GIBCO), 100 U/mL penicillin (GIBCO), 100 mg/mL streptomycin (GIBCO), 0.05 mM β-mercaptoethanol (Sigma-Aldrich), and 2 mM L-glutamine (GIBCO), at a density of 3–9 × 105 cells/mL at 37°C in 5% air 95% CO2. Extracts from the three thyme species were fractionated using a two-cascade depressurized system and samples were collected in each of the two (separators 1 and 2) separators. The action of thyme fractions appears to involve the expression of the proinflammatory cytokine: TNF-α, IL-6, and IL-1β and the anti-inflammatory cytokine IL-10. Thymol and carvacrol in essential oils are often quantified by gas chromatography (GC) technique but in this work, a … At 48 hours of incubation, all treatments, except Thymus hyemalis S2, caused reduction in gene expression. Naturally protective with a wonderful herby fragrance, this thyme essential oil is steam distilled from the leaves. The HPLC system consisted of ACE C(18) column and an isocratic acetonitrile:water (50:50) as the mobile phase which was kept at a flow rate of 1 ml/min. The use of the extracts and herb of thyme plants as antibacterial, antioxidant, natural food preservative and in cosmetic industry has increased the economic importance of this medicinal crop worldwide. Thymus zygis and Thymus vulgaris increased significantly IL-10 secretion of activated cells, with both fractions and at any of the concentrations used. 8600 Rockville Pike Fleurs roses. and incubated with Phorbol 12-Myristate, 13-Acetate (PMA) 100 ng/mL (Sigma-Aldrich) for 48 h in FBS-free medium. Epub 2018 May 22. 48-hour treatments with higher concentrations of the fractions induced an increase on IL-10 secretion; the rest concentrations did not induce significant increases. 2, 2019 pp. The antimicrobial properties of essential oils have been known for many years (Williams et al. Potentially irritating. 4041-4044 e-ISSN:2581-6063 (online), ISSN:0972-5210 MACERATION TECHNIQUES EXTRACTION OF THYMUS VULGARIS AND LAUREL (LAURUS NOBILIS) LEAVES WITH ANTIBACTERIALSTUDY Semma H. Shalaal1, Afrah T. Halail2, Fadil M. Hamed 3 and Bassam A. Hassan4* 1*, 3 & 4Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Thi-Qar University, Iraq. Salehi B, Mishra AP, Shukla I, Sharifi-Rad M, Contreras MDM, Segura-Carretero A, Fathi H, Nasrabadi NN, Kobarfard F, Sharifi-Rad J. Phytother Res. 24-hour treatment with any of the fractions induced an increase on IL-10 secretion in a dose-dependent manner; higher fraction concentration induced higher IL-10 secretion. Trouvé à l'intérieur â Page 841Ind Crops Prod 2006;24:253-263 22 Golmakani M-T, Rezaei K. Comparison of microwave-assisted hydrodistillation with the traditional hydrodistillation method in the extraction of essential oils from Thymus vulgaris L. Food Chem 2008 ... SDS 2.2.2 software was used. Thyme oils have also been described as a strong bactericide against Gram-positive and Gram-negative bacteria and also as a bronchospasmolytic [9, 10]. Total RNA isolated from THP-1 cells was quantification of IL-1β, IL-6, IL-10, TNF-α, 18sRNA, and GAPDH gene expression using real-time PCR. Total RNA from THP-1 cells was isolated using the Trizol reagent from Invitrogen. Among different species of Thymus, Thymus vulgaris is used more than other species in therapeutic dosage forms. Trouvé à l'intérieur â Page 559J Burn Care Rehabil 24(6):395â399 El-Nekeety AA, Mohamed SR, Hathout AS, Hassan NS, Aly SE, Abdel-Wahhab MA (2011) Antioxidant properties of Thymus vulgaris oil against aflatoxin-induce oxidative stress in male rats. Oven temperature programming was 60°C isothermal for 4 min, increased to 64°C at 1°C/min, and then increased to 106°C at 2.5°C/min. Cytokines exhibit both beneficial and pathologic effects on their target cells and are produced by many cell types [32]. Our results are concordant with Sharma et al. It is a bushy, woody based shrub, 10 to 40 cm high with small and highly aromatic gray-green oval leaves containing numerous small glands with clusters of pink or purple flowers. Trouvé à l'intérieur â Page 57... dioica Avena sativa Mentha x piperita Rosmarinus officinalis Salvia officinalis Thymus vulgaris Nasturtium officinale Hamamelis virginiana Achillea millefolium 6 60 Naturally Lustrous Hair Recipes: Splendid Lavender & Grapefruit ... Red Thyme is a Thymus zygis but the method of extraction creates concentrations of thymol that are excessive for frequent use or for use with children. Trouvé à l'intérieur â Page 101(7.5%), p-cymene (6.7%), linalool (5.5%) and 1,8-cineole (1.8%) (Thymus vulgaris F.) and by p-cymene (17.2%), ... account in the evaluation of the antifungal activity of the EOs studied with the employed disk diffusion assay technique. The inlet pressure of the carrier gas was 57.5 KPa. There is a decrease in IL-1β secretion with the increase of fraction concentration, although this IL-1β decrease is only significant till 25 μg/mL incubations. Some of them are involved in proatherogenic processes, such as upregulation of adhesion molecules on the endothelial cells, while others were proved to have an antiatherogenic role like attenuating the differentiation of monocytes in macrophages. Helium, 99.996%, was used as a carrier gas at a flow of 1 mL/min. The chromatographic method was to be based on the previously described by Jordán et al. Blumenthal M. Newton:: Integrative Communications; 2000. is a medicinal and culinary herb from the Southern European region known for its anti-infective, cardioprotective, gastroprotective, anti-inflammatory, and immunomodulatory . Then, cells were incubated with the corresponding thyme extract diluted in FBS-free medium, for 24 or 48 hours at 37°C in 5% CO2. However, too many little is known about thyme anti-inflammatory activity. Cells were pelleted via centrifugation and assessed for viability using the Trypan-blue exclusion method. Trouvé à l'intérieur â Page 80Terminologie : Noms latins : - Thymus vulgaris L. Noms français : - Thym commun , Thym vulgaire , Farigoule pour les ... Mis à part le thym vulgaire , décrit dans cette fiche , la plus répandue et la plus intéressante du point de vue ... This paper aims to evaluate the inhibitive action of the corrosion of mild steel in sulphuric acid solutions by ethanol extracts of Thymus vulgaris (TYV), Xylopia aethiopica (XYA) and Zingiber officinale (ZGO) as eco-friendly and non-toxic mild-steel corrosion inhibitors in H2SO4 solutions.,Ethanol extracts of TYV leaves, XYA fruits and ZGO roots were used as inhibitors in various corrosion tests. IL-6 secretion was reduced significantly when activated cells were incubated with any of the fractions of either Thymus zygis or Thymus vulgaris at a concentration of 15 μg/mL or higher. While the inhalation of Thymus vulgaris L. essential oil (EO) is commonly approved for the treatment of mild respiratory infections, there is still a lack of data regarding the antimicrobial activity and chemical composition of its vapours. Management strategies using natural products, e.g., plant extracts, might be alternatives for control of this pest. The essential oils obtained from Thymus vulgaris L. harvested at four ontogenetic stages were evaluated for their biological activity and chemical composition. Changes on production and gene expressions were dose dependent and according to the thyme content of each species. Both fractions of Thymus zygis or Thymus vulgaris induced similar reduction of IL-1β. Read the winning articles. Rodríguez-Solana R, Salgado JM, Domínguez JM, Cortés-Diéguez S. Phytochem Anal. In the case of Thymus vulgaris L. the effect of different drying techniques (natural way, convective drying at 30 °C, 40 °C and 50 °C and lyophilization) were investigated on the quantity and quality parameters of the final product analyzed by GC-MS and sensory profile methods.Measured essential oil amounts were between 0.69 ml/100 g and 1.84 ml/100 g calculated on the dry weight basis. Also, thymol and carvacrol prevent autoxidation of lipids [37]. The most crucial thing you can do to save a browning thyme plant is to cut back on watering to once a week. OF Thymus vulgaris THROUGH FOLIAR APPLICATION OF SALICYLIC ACID UNDER WATER STRESS . Carvacrol also possesses antimicrobial, antifungal, and antioxidant activities [20–22], as well as antimutagenic and anticarcinogenic effects [23]. 1998) and those from popular commercially available aromatic plants such as Melaleuca alternifolia (tea tree), Thymus vulgaris (thyme), Mentha piperita (peppermint) and Rosmarinus officinalis (rosemary) have been used extensively to treat bacterial . The obtained essential oil was dried over sodium sulphate and stored at 4 C in dark vials until tested. The GC system consisted of flame ionization detector (FID) and CP-SIL 8 column. 2018 Sep;32(9):1688-1706. doi: 10.1002/ptr.6109. Several natural compounds are known for their beneficial properties to some diseases or their derived complications and particularly concerning anti-inflammatory effects. Mixtures of n-heptane, ethyl acetate, methanol, and water (5:2:5:2 and 4:1:4:1 v/v), allowed purification of eugenol, 1-octen-3- ol, borneol, thymol, … Industrial Crops and Products. Agricultural practices have an influence on growth and yield of thyme plants. The model was constructed using 30 samples of authenticated Thymus vulgaris and challenged with 20 samples of different botanical origins. AyyadWajeeh Raaof 1,, Zahraa Abdul-Elah Al-Naqqash 2, Abdul-Latif M. Jawad 1, Salah M. Muhsan 3. Thyme essential oil contains the powerful antioxidant thymol that supports the immune system. Aliksir inc. 1040, chemin du Roy, Grondines, G0A 1W0 (1-866-596-3406), www.aliksir.com, [email protected] The absorbance of each well was then read at 540 nm using a microplate reader (Sunrise Remote, Tecan). Total RNA was then dissolved in 15 μL of DEPC H2O, incubated at 55°C for 10 minutes, and stored at −80°C for future use. Trouvé à l'intérieur â Page 117For basophile holm oaks , the following species were defined : Quercus coccifera , Thymus vulgaris , Genista scorpio , Rhamnus lycioides , Lithospermum fruticosum , Rosmarinus officinalis , Thymus zygis , Cistus albidus , and Salvia ... For example, it has been reported that thyme oil reduces NO production in J774A.1 murine macrophages [11]. After incubation, samples were centrifuged at 12000 g, 4°C for 10 minutes. Thymus vulgaris. 19 No. Supercritical fluid extraction (SFE) is considered an attractive extraction method when compared to conventional techniques such as steam distillation or Soxhlet extraction because it avoids solute contamination with solvent residues and the degradation of thermolabile compounds [25]. Trouvé à l'intérieurSYMBOLIC MEANINGS: Everlasting friendship; Friendship; Live for me; Unchanging; True friendship; Unchanging affection; Unchanging friendship; ~ ⢠~ 881 Thymus vulgaris | Common Thyme | English Wild Thyme| Garden Thyme | Thyme ... The plates were washed and incubated for 30 min in the dark with substrate solution. Trouvé à l'intérieur â Page 142La composition de ces formules est la suivante : 1 - Hypericum perforatum L. ( Herba hyperici ) Mentha piperita Huds ( Folia menthae ) Thymus vulgaris L. ( Herba thymi ) Eucalyptus Globulus Labill ( Folia eucalyptus ) Matricaria ... Relative quantification (RQ) determinates the change in expression of a nucleic acid sequence relative to a control. Following homogenization, samples were left to rest at room temperature for 5 minutes. A gel-like pellet was formed and the isopropyl alcohol was removed. The development of acute bronchitis is often preceded by symptoms of upper . As a cellular model of inflammation/atherogenesis, we use human macrophages derived from THP-1 monocytes and activated by oxidized LDLs. It was carried out as positive standard control [27, 28]. Oven temperature was then increased from 106°C to 130°C at 1°C/min, then to 200°C at 5°C/min, and then to a final temperature of 250°C/min at 8°C/min which was kept constant for 10 min. Supernatants were centrifuged at 12,000 rpm to remove debris and then stored at −80°C until cytokine analysis. Afterwards, the supernatant was frozen and RNA isolated. This process becomes macrophage into foam cells that characterize the early atherosclerotic lesion [4]. These results may suggest that essential oils from thyme extracts could be used as novel options for treatment of chronic diseases based on inflammatory processes. Thymus vulgaris (thyme) is commonly used in folk medicine for several therapeutic purposes. Regarding IL-1β release at 24 hours, treatment of activated cells with any of the thyme fractions induced a decrease of this cytokine secretion in a dose-dependent manner (Figure 3(b)). Trouvé à l'intérieur â Page 637... distans thym Thymus vulgaris thousand - leaf Achillea millefolium thym commun Thymus vulgaris three faces in a hood Viola tricolor thym vrai Thymus vulgaris three - leaved caper Crataeva religiosa thym vulgaire Thymus vulgaris three ... Free shipping to Canada on orders of 100$ or more / 10% off orders over 200$, Aliksir inc. 1040, chemin du Roy, Grondines, G0A 1W0 (1-866-596-3406), www.aliksir.com, [email protected]. Both IL-1 and IL-6 enhance the inflammatory process. Rests of the compounds were identified by comparison with the mass spectra from Wiley 229 library and by their linear retention index. As a symbol of courage, the image of thyme is used in Northern Europe until the Great Crusades. The optical density of formazan formed in control cells (without treatment with extract) was taken as 100% viability. Thymol and carvacrol in essential oils are often quantified by gas chromatography (GC) technique but in this work, a validated and reliable high performance liquid chromatography (HPLC) method has been developed for the analysis of these two components in T. vulgaris essential oil. Sweetpotato whitefly, Bemisia tabaci (Gennadius), is an important pest of many crops, with an extremely wide host range. After 48 hours, incubations with these two fractions reduced IL-1β secretion to nonactivated basal levels in the same manner as Thymus zygis and Thymus vulgaris did. antique medical scientific illustration high-resolution: thymus - thymus stock illustrations Some of these mediators are involved in proatherogenic processes, such as interleukin- (IL-) 1, IL-6, and Tumor Necrosis Factor alpha (TNF-α) that involve in the upregulation of the molecular adhesion on the endothelial cells. Growth kinetics were measured with an inoculation of Verticillium dahliae and identified by GC-MS. Total volatile phenolic constituents were measured. values less than 0.05 were considered significant. The current study was designed to investigate the insecticide role of volatile constituents produced from cell suspensions of T. vulgaris and O. basilicum against R. ferrugineus. Thymus vulgaris L., garden thyme, has a long history of use by humans. Main compounds presented in the supercritical extracts of the three thyme varieties were 1,8 cineole, thymol, camphor, borneol, and carvacrol. Astier_Demarest_FT_FR_THYM_LINALOL_BIO_CE_Thymus_Vulgaris_.pdf 0.00 KB. A. Lemli, “Spasmolytic activity of the flavonoids from, T. Essawi and M. Srour, “Screening of some Palestinian medicinal plants for antibacterial activity,”, E. Vigo, A. Cepeda, O. Gualillo, and R. Perez-Fernandez, “In-vitro anti-inflammatory effect of Eucalyptus globulus and, M. Hudaib, E. Speroni, A. M. Di Pietra, and V. Cavrini, “GC/MS evaluation of thyme (, H. Tian and D.-M. Lai, “Analysis on the volatile oil in Origanum vulgare,”, P. C. Braga, M. Dal Sasso, M. Culici, T. Bianchi, L. Bordoni, and L. Marabini, “Anti-inflammatory activity of thymol: inhibitory effect on the release of human neutrophil Elastase,”, Y. Suzuki and H. Furuta, “Stimulation of guinea pig neutrophil superoxide anion-producing system with thymol,”, R. Aeschbach, J. Löliger, B. C. Scott et al., “Antioxidant actions of thymol, carvacrol, 6-gingerol, zingerone and hydroxytyrosol,”, N. Didry, L. Dubreuil, and M. Pinkas, “Activity of thymol, carvacrol, cinnamaldehyde and eugenol on oral bacteria,”, M. Segvi Klari, I. Kosalec, J. Masteli, E. Piecková, and S. Pepeljnak, “Antifungal activity of thyme (, S. Fujisawa and Y. Kadoma, “Effect of phenolic compounds on the polymerization of methyl methacrylate,”, B. Bozin, N. Mimica-Dukic, I. Samojlik, and E. Jovin, “Antimicrobial and antioxidant properties of Rosemary and Sage (, K. Horosova, D. Bujnakova, and V. Kmet, “Effect of oregano essential oil on chicken Lactobacilli and E. coli,”, M. H. Alma, A. Mavi, A. Yildirim, M. Digrak, and T. Hirata, “Screening chemical composition and, C. C. Arcila-Lozano, G. Loarca-Pina, and S. Lecona-Uribe, “Oregano: properties, composition and biological activity,”, M. P. Martínez-Montiel and M. T. Muñoz-Yagüe, “Biologic therapies for chronic inflammatory bowel disease,”, P. F. S. 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Liu, and S. T. Chang, “Study on the antiinflammatory activity of essential oil from leaves of cinnamomum osmophloeum,”, S. Sosa, G. Altinier, M. Politi, A. Braca, I. Morelli, and R. Della Loggia, “Extracts and constituents of Lavandula multifida with topical anti-inflammatory activity,”, J. Mastelić, I. Jerković, I. Blažević et al., “Comparative study on the antioxidant and biological activities of carvacrol, thymol, and eugenol derivatives,”, J. M. Prieto, P. Iacopini, P. Cioni, and S. Chericoni, “In vitro activity of the essential oils of Origanum vulgare, Satureja montana and their main constituents in peroxynitrite-induced oxidative processes,”, N. V. Yanishlieva, E. M. Marinova, M. H. Gordon, and V. G. Raneva, “Antioxidant activity and mechanism of action of thymol and carvacrol in two lipid systems,”, U. R. Juergens, T. Engelen, K. Racke, M. Stober, A. Gillissen, and H. 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